Therapeutic Drug Monitoring of Antineoplastic Drugs

Methotrexate is a competitive inhibitor of dihydrofolate reductase, a key enzyme for biosynthesis of nucleic acid. The cytotoxic activity of this drug was discovered in 1955. The use of leucovorin to rescue normal host cells has permitted the higher doses of methotrexate therapy in clinical practice. Methotrexate is used in the treatment of acute lymphoblastic leukemia (ALL), osteogenic sarcoma, brain tumors, and carcinomas of the lung. Most of the toxicities of this drug are related to serum...