Streptococcal Cell Wall Arthritis

Cure Arthritis Naturally

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unit 15.10

Streptococcal cell wall (SCW) arthritis in rats is an experimentally-induced inflammatory model with many features that resemble rheumatoid arthritis (RA) in humans. The model is used widely in studies of genetics, pathogenesis, and therapy of acute and chronic inflammation. Arthritis is induced in an appropriately susceptible inbred rat strain (Table 15.10.1) by an intraperitoneal injection of peptidoglycan-polysaccharide polymers (PG-PS), which are isolated from the sonicated cell walls of Streptococcus pyogenes, Group A, D58 strain. In addition to arthritis, intraperitoneal injection of Group A SCW PG-PS may also induce hepatic, splenic, and peritoneal granulomas. SCW PG-PS-induced responses are considered representative of several naturally occurring inflammatory disorders.

In this unit, Lewis rats, a highly susceptible inbred rat strain, are injected with an aqueous suspension of Group A SCW PG-PS and observed daily for the development of arthritis for the first 6 days and then every 2 to 3 days for 6 to 8 weeks (see Basic Protocol). The resulting arthritis is biphasic. An acute phase typically develops within 48 hr, followed 10 to 21 days later by a chronic phase which persists for months. Support protocols are included for preparing Group A PG-PS (see Support Protocol 1) and measuring PG-PS concentration (see Support Protocol 2).

Table 15.10.1 Relative Susceptibility to Chronic SCW Arthritis in Inbred Rat Strains3

Incidence and severity

Strains6

High

LEW/N, LOU/MN, LA/N, RHA/N, NSD/N, M520/N

Intermediate

A28807/N, BN/N, ODU/N, MK/N, MNR/N, SHR/N

Low

BUF/N, ACI/N, CAR/N, WKY/N, F344/N

aFemale rats, -100 g at time of treatment, treated with 60 |g of cell wall rhamnose/g body weight. From Wilder et al. (1982).

^For extensive information about commonly used rat strains, see APPENDIX 1H.

aFemale rats, -100 g at time of treatment, treated with 60 |g of cell wall rhamnose/g body weight. From Wilder et al. (1982).

^For extensive information about commonly used rat strains, see APPENDIX 1H.

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Arthritis Joint Pain

Arthritis Joint Pain

Arthritis is a general term which is commonly associated with a number of painful conditions affecting the joints and bones. The term arthritis literally translates to joint inflammation.

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  • temshe
    What drugs can be used in streptococcal cell wall induced arthritis model?
    9 months ago

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