Measurement Of B Cell Responses To Cii In

In addition to visual inspection of the animals (see Support Protocol 4), another means of evaluating the effectiveness of the immunization with CII is measurement of humoral and cellular immune responses. Antibody titers correlate strongly with the presence or absence of arthritis within most strains, but may vary among individual strains. CII-spe-cific antibody can be easily measured using a standard indirect ELISA (see unit 2.1) in which the CII is absorbed to the plate. T cell responses to CII can be evaluated both by proliferation assays and by the production of IFN-y using a solid-phase ELISA (see Support Protocol 8).

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