Induction Of Collageninduced Arthritis In The Mouse

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The mouse model of collagen-induced arthritis (CIA) is particularly advantageous for study, given the vast number of inbred strains available and their corresponding immunologic reagents. Disease induction is more complex than that in other autoimmune disease models—e.g., experimental autoimmune encephalomyelitis (EAE; unit 15.1)—in that CIA requires both the activation of T cells and the production of antibodies specific for both the immunogen and the autoantigen, murine CII.

This protocol is for the DBA/1JLacJ strain of mouse; see Background Information for discussion of susceptibility of various mouse strains to CIA.

Materials

Chick or bovine native CII (see Support Protocol 1 or purchase from Sigma or Chondrex), a1(II) chains (see Support Protocol 2), or CB11 fragment of CII (see Support Protocol 3) 10 mM acetic acid, filter sterilized with 0.2-^m filter Incomplete Freund's adjuvant (IFA; e.g., Difco)

Mycobacterium tuberculosis (strain H37Ra; heat-killed; available by writing to Ministry of Agriculture, Fisheries, and Food, Central Veterinary Laboratory, Weybridge, Surrey, United Kingdom) DBA/1JLacJ mice (Jackson Labs)

Mortar and pestle

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