In this protocol, adjuvant arthritis (AA) is induced in Lewis rats in the classical manner using ground, heat-killed Mycobacterium tuberculosis H37Ra suspended in incomplete Freund's adjuvant (IFA; see Support Protocol). Disease development is monitored daily during the critical period (days 10 to 35 post immunization) using one or more possible techniques. The success rate of the procedure should approach 100%.
10 mg/ml heat-killed Mycobacterium tuberculosis (strain H37Ra) suspended in incomplete Freund's adjuvant (IFA; see Support Protocol) Lewis rats, 6 to 12 weeks old and pathogen free
1-ml glass syringe 20- and 25-G needles Scale
Restraining device for rats Wet cotton
Additional reagents and equipment for animal care, handling, and restraint
daily inspection, scoring, and weighing daily inspection, scoring, and weighing
10-17 25-35 40-42
Day post immunization
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