Induction Of Adjuvant Arthritis

Cure Arthritis Naturally

Beat Arthritis Naturally

Get Instant Access

In this protocol, adjuvant arthritis (AA) is induced in Lewis rats in the classical manner using ground, heat-killed Mycobacterium tuberculosis H37Ra suspended in incomplete Freund's adjuvant (IFA; see Support Protocol). Disease development is monitored daily during the critical period (days 10 to 35 post immunization) using one or more possible techniques. The success rate of the procedure should approach 100%.

Materials

10 mg/ml heat-killed Mycobacterium tuberculosis (strain H37Ra) suspended in incomplete Freund's adjuvant (IFA; see Support Protocol) Lewis rats, 6 to 12 weeks old and pathogen free

1-ml glass syringe 20- and 25-G needles Scale

Restraining device for rats Wet cotton

Additional reagents and equipment for animal care, handling, and restraint

daily inspection, scoring, and weighing daily inspection, scoring, and weighing

10-17 25-35 40-42

Day post immunization

Was this article helpful?

0 0
Natural Arthritis Relief

Natural Arthritis Relief

Natural Arthritis Relief details a unique method of reversing Rheumatoid Arthritis Symptoms by removing numerous arthritis triggers as well as toxins using a simple 5 step natural process.

Get My Free Ebook


Post a comment