Induction And Clinical Evaluation Of Arthritis

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In this protocol, arthritis is induced in Lewis rats by an intraperitoneal injection of an aqueous suspension of Group A PG-PS (SCW), prepared as described in Support Protocol 1. Disease development is monitored by visual inspection or measurement of ankle diameters for periods of 6 to 8 weeks. Incidence of disease should approach 100%.

Materials

Lewis rats, female, 6- to 12-weeks-old or 100 to 200 g, specific-pathogen-free

(Harlan Bioproducts) 3 to 6 mg rhamnose/ml 10S PG-PS suspension in sterile 0.85% NaCl (see Support

Protocol 1; alternatively, purchase from Lee Laboratories) 0.85% NaCl, sterile

Scale appropriate for weighing rats Calipers for measuring ankle diameter (optional) Sonicator: Branson Sonifier 350 (VWR) or equivalent 1-ml syringes and 23-G needles

Additional reagents and equipment for animal handling and restraint (unit 1.3), animal identification (unit 1.5), and intraperitoneal injection of rats (unit 1.6)

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Osteoarthritis

Osteoarthritis

Thank you for deciding to learn more about the disorder, Osteoarthritis. Inside these pages, you will learn what it is, who is most at risk for developing it, what causes it, and some treatment plans to help those that do have it feel better. While there is no definitive “cure” for Osteoarthritis, there are ways in which individuals can improve their quality of life and change the discomfort level to one that can be tolerated on a daily basis.

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