New Zealand Strains

The most well-described New Zealand (NZ) lupus strains include the NZ black (NZB) x NZ white (NZW) F1 and NZ mixed (NZM). Their initial origins are somewhat obscure, but NZB appears to have originated in the late 1940s during inbreeding of outbred stocks at the University of Otago School of Medical Sciences, New Zealand, during efforts to develop mouse strains for cancer research (17-19), and NZW was derived separately in 1952 during selective Exudative, proliferative F > D > M > MP...

Stanford L Peng Summary

Murine models of systemic lupus erythematosus provide fertile research systems for the pathogenesis and therapy of systemic autoimmune disease. Their phenotypes span the broad range of clinical manifestations of human lupus and consist of both spontaneous and experimentally induced disease in both inbred and targeted mutant animals. This chapter contrasts the clinical characteristics of these various models, providing an outline for the use and analysis of these in vivo autoimmune systems....

Murphys Recombinant LargeMpJ

Murphy's recombinant large (MRL) Mp mice developed from efforts in the 1960s to develop inbred mouse strains for the study of obesity (20,99), but they were found to develop autoantibodies and end-organ disease highly reminiscent of human SLE. They currently are estimated to consist of 75 LG J, 12.6 AKR, 12.1 C3H Di, and 0.3 C57BL 6 lineages. During the 12th brother-by-sister intercross, the spontaneous lymphoproliferation (Ipr) mutation developed, associated with massive lymphadenopathy and...

Methods 31 Real Time PCR

After informed consent, heparinized blood (in 10-mL green-top tubes) is obtained by venipuncture from each individual. 1. Blood is mixed with an equal amount of HBSS and layered carefully over 12 mL of Ficoll-Paque Plus in a 50-mL Falcon tube. Spin at 700g for 20 min with brakes off (gradient centrifugation). 2. With a Pasteur pipet, remove carefully the PBMC cell layer from the interface between Ficoll and diluted plasma. Try to avoid obtaining too much Ficoll. Spin at 300g for 10 min. 3. Wash...

Mitochondrial Dysfunction in Lupus T Cells

SLE T cells exhibit both defective AICD and enhanced spontaneous apoptosis Table 1 . Coordinate mitochondrial hyperpolarization and ATP depletion play key roles in abnormal T-cell death of patients with SLE 36 , AWm and ROI levels as well as cytoplasmic pH are elevated in patients with SLE in comparison to healthy or controls with rheumatoid arthritis 36,37 . Baseline mitochondrial hyperpolarization and ROI levels correlated with diminished GSH levels, suggesting increased utilization of...

Info

Liver antibody-mediated targeting cytochrome P450 Although the causes of autoimmune diseases have not been defined, independent lines of evidence have implicated environmental factors and genetic determinants of the host 2 . As shown in Table 1, polymorphisms of HLA molecules that regulate antigen presentation 3 and complement deficiency states 4 have been identified as inherited factors influencing disease susceptibility. Concordance rates for autoimmune diseases such as SLE, IDDM, RA, and MS...